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KMID : 0388220040110010025
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Journal of the Korean Rheumatism Association
2004 Volume.11 No. 1 p.25 ~ p.36
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Reciprocal Activation of Fibroblast-like Synoviocyte and Type Ⅱ Collagen-reactive T cell in Rheumatoid Arthritis
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Abstract
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Objective: To investigate the interaction between type 11 collagen (CII)-reactive T cell and fibroblast-like synoviocyte in rheumatoid arthritis (RA).
Methods: Peripheral blood T cells from RA patients were cultured with bovine C11 and analyzed by flow cytometry. After co-culture with C11-reactive T cells and fibroblast-like synoviocytes (FLS), the expression of cytokines (IL-15 and TNF- a from FLS, IFN- y and IL-17 from C11-reactive T cells) were determined by ELISA and RT-PCR.
Results: C11-reactive T cells expressed CD69, one of the early activation markers, and produced significant amount of IFN- y, and proliferated. IL-15 and TNF- a expression from FLS were significantly elevated when co-culture with CII-reactive T cells and inhibited by physical int6rruption of cell-to-cell contact or anti-CD40 antibody. IFN-y and IL-17 expression from CII-reactive T cells were also significantly elevated when co-culture with FLS and inhibited by anti-IL-15 monoclonal antibody.
Conclusion: CII-reactive T cells can activate FLS to secret proinflammatoy cytokines and interactions between these two cells drive further activation of each other. These data suggest that CII-reactive T cell may play a important role in pathogenesis of RA.
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KEYWORD
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